[3PS-092]
Multilamellar Lipid Nanovaccines Incorporating Clumping Factor A and Dual TLR Adjuvants for Enhanced Anti–Staphylococcus aureus Immunity
발표자안동우 (KAIST)
연구책임자남윤성 (한국과학기술원)
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious clinical challenge due to antibiotic resistance and the absence of effective vaccines. We present a biomimetic nanovaccine platform based on structurally reinforced multilamellar lipid encapsulating the fibrinogen-binding N23 domain of clumping factor A (ClfAN23) together with dual Toll-like receptor adjuvants. Incorporating ceramide ENP enables the formation of robust multilamellar architectures with markedly improved stability against lyophilization, repeated freeze-thaw cycles, and serum exposure. Optimized surface presentation of MPLA and CpG-Chol synergistically enhances dendritic cell maturation, co-stimulatory molecule expression, and proinflammatory cytokine secretion. In vivo immunization elicits strong antigen-specific humoral responses and Th1/Th17-skewed T cell immunity. This work was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) (RS-2025-00516761).