[3PS-253]
Isolation of Therapeutic Extracellular Vesicles Using Nanoporous Membranes with Uniform Nanopores
발표자이경원 (부산대학교)
연구책임자양승윤 (부산대학교)
Abstract
Extracellular vesicles (EVs) are nanosized particles secreted by cells, reflecting the characteristics of their mother cells. Mesenchymal stem cell (MSC)-derived EVs show potential for regenerative wound healing and modulating immune responses. However, traditional isolation methods suffer from limited yield and purity. This study reports a two-step tangential flow filtration (TFF) system utilizing track-etched membranes with uniform cylindrical nanopores (50 and 200 nm) to isolate small EVs (sEVs) via a size-exclusion mechanism. Comparative analysis reveals that this nanoporous membrane-based TFF (Nano-TFF) system achieves a separation efficiency exceeding twofold and a purity surpassing 90% over existing ultrafiltration systems. The efficacy of these sEVs was validated in a wound animal model, where sEV-loaded dressings enhanced recovery. The Nano-TFF system provides precise, high-efficiency separation for various bioactive agents requiring high-purity isolation