Peptide-imprinted polymer-based indirect competitive ELISA for detection of TTX via initiated chemical vapor deposition (iCVD)
발표자
조채환 (한국과학기술연구원)
연구책임자
성혜정 (한국과학기술연구원)
공동저자
조채환 (한국과학기술연구원), 박종필 (중앙대학교), 성혜정 (한국과학기술연구원)
초록
내용
Tetrodotoxin (TTX) is a potent neurotoxin and a significant concern for public health and environmental safety. Enzyme-linked immunosorbent assay (ELISA) is widely used for rapid TTX detection; however, conventional antibody-based competitive assays are limited by high cost, low stability, and assay complexity. In this study, we present a peptide-imprinted polymer (PIP)-based indirect competitive ELISA (ic-ELISA) for TTX detection. TTX-binding peptide (TTX BP) was identified by biopanning and used as a template to fabricate a PIP on microplate wells via initiated chemical vapor deposition (iCVD) using a HEMA-co-EGDMA copolymer. Competitive binding between a biotinylated TTX-binding signal peptide (TTX SP) and TTX was quantified using streptavidin–HRP, resulting in a concentration-dependent decrease in absorbance. The developed PIP-based ic-ELISA exhibited excellent linear correlation with TTX concentration, demonstrating its potential as a cost-effective and robust alternative for TTX detection. Acknowledgement: This research was supported by National R&D Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No. RS-2025-00517552), KIST research programs (2E33682, 2E33712, 2E3375H), and Ministry of Food and Drug Safety (Grant No. 20163MFDS641).