The Polymer Society of Korea

김지성 (포항공과대학교)

이기라 (포항공과대학교)

Nanoparticles with spatially programmed surface patches enable directional self-assembly, but scalable fabrication methods are limited. We present a recyclable DNA origami lattice platform for programmable patchy gold nanoparticle fabrication. Octahedral DNA origami frames with internal binding sites encapsulate AuNPs and generate patches via molecular stamping and strand displacement. Assembling the frames into a three-dimensional simple cubic lattice allows efficient release and separation of patchy nanoparticles by centrifugation. We fabricate particles with one, two, and four patches, which assemble into dimers, chains, and two-dimensional networks, demonstrating valence-controlled assembly. The origami lattice remains intact after release and can be regenerated for multiple fabrication cycles, as confirmed by SAXS and TEM. This approach provides a reusable platform for programmable nanoparticle assembly.