Polydopamine-Mediated Hydrogel Adhesion Enables a PDMS 3D ALI Skin Scaffold
발표자
윤별 (경상국립대학교)
연구책임자
강유경 (경상국립대학교)
공동저자
윤별 (경상국립대학교), 김경은 (경상국립대학교), 강유경 (경상국립대학교)
초록
내용
3D skin scaffolds are crucial alternatives to animal testing, yet maintaining long-term structural integrity and user-friendly operation remains challenging. This study presents a human skin model based on 3D-printed polydimethylsiloxane (PDMS). By utilizing PDMS to perform air-liquid interface (ALI) culture, we simplified the culture procedure and designed the system to be suitable for large-scale production and automated processing. To address hydrogel contraction issues during long-term culture, polydopamine surface coating was applied to enhance adhesion between the scaffold and hydrogel. Through co-culture of endothelial cells, fibroblasts, and keratinocytes, we successfully recapitulated skin-like features including vascularized dermal structure and stratified epidermal structure. This model can be applied to drug evaluation and regenerative medicine.