Bioactive hydrogels are promising biomaterials for tissue regeneration. Recently, the incorporation of exosomes has garnered attention for enhancing therapeutic efficacy and tissue repair. While animal-derived exosomes are prevalent, they have limitations such as high immunogenicity and significant costs. Conversely, plant-derived exosomes offer low immunogenicity and cost-effectiveness. Here, we developed perilla leaf-derived exosome-releasing hydrogels (Perex-Gels) via thiol-ene, amine-ene reactions, and disulfide bonds. Our hydrogels formed a network within 15-17 seconds and provided adequate mechanical strength (155-305 Pa) for stability in vivo. Additionally, Perex-Gels demonstrated a sustained release of Perex over 24 hours, both in vitro and in vivo. Notably, Perex-Gels accelerated wound healing by modulating M2 macrophage polarization and promoting angiogenesis in vivo. Overall, Perex-Gels exhibit significant potential as innovative bioactive materials for tissue regeneration