DNA hydrogels are promising for biomedical use due to their programmability and biocompatibility, but their application in load-bearing environments is limited by insufficient mechanical strength. Conventional reinforcement strategies frequently rely on synthetic additives or chemical crosslinkers that can negatively impact intrinsic biological compatibility.
To address this challenge, we introduce a methodology for fabricating mechanically robust DNA hydrogels specifically tailored for implantable applications. By employing a protein-mediated reinforcement process, we successfully developed hydrogels with adjustable stiffness and significantly enhanced physical durability. These materials demonstrate stable mechanical properties under physiological conditions, effectively mimicking the biomechanics required for structural support. This platform provides the structural integrity needed for long-term implants while maintaining their biocompatibility.