Immobilization of Angiogenic Peptides on Mesenchymal Stem Cells via Ex Vivo Cell Surface Engineering for Liver Fibrosis Attenuation
발표자
박희원 (국립암센터)
연구책임자
김교범 (동국대학교)
공동저자
박희원 (국립암센터), 김성준 (국립암센터), 김교범 (동국대학교)
초록
내용
Placenta-derived mesenchymal stem cells(PDMSCs) have emerged as a promising modality for liver diseases due to their therapeutic efficacy. To enhance the potential, ex vivo cell surface engineering was employed. The peptide, WKYMVm was immobilized onto the PDMSC surface using lipid–polyethylene glycol biomaterial via hydrophobic interaction. WKYMVm shows specific affinity for FPR2, inducing angiogenesis and tissue repair. We hypothesized that immobilized peptide could be delivered to injured liver through engineered PDMSCs and activate neighboring PDMSCs or damaged hepatocytes, leading to effective tissue recovery with reduced peptide usage. In vitro and in vivo studies demonstrated that engineered PDMSC enhanced angiogenic activity and promoted liver recovery compared to naïve PDMSCs. Overall, this study highlights peptide-based stem cell surface engineering as a promising strategy for liver therapy.