Nucleic acid-based micelle incorporated with USE1-silencing siRNA for lung cancer therapy
발표자
이소윤 (국립부경대학교)
연구책임자
곽민석 (국립부경대학교)
공동저자
이소윤 (국립부경대학교), 김해주 (부산대학교), 곽민석 (국립부경대학교)
초록
내용
UBA6-specific E2 conjugation enzyme 1 (USE1) is frequently overexpressed in lung cancer patients and plays a critical role in tumor progression. In this study, we aimed to develop USE1-targeted therapeutics based on RNA interference (RNAi). We designed a lipid-modified DNA carrier, U4T, composed of four consecutive dodec-1-ynyluracil nucleobases, to enhance the cellular uptake of USE1-targeting siRNA. U4T spontaneously assembles into micelles, and USE1-silencing soft spherical nucleic acid aggregates (siSNAs) are readily formed through simple base pairing with siRNA. Treatment with siSNA effectively suppressed tumor growth in vivo and inhibited proliferation, migration, and invasion of lung cancer cells. Moreover, siSNA induced G1-phase cell cycle arrest and apoptosis. Notably, siSNA exhibited efficient cellular penetration without the use of cationic transfection agents, demonstrating its potential as an RNAi-based therapeutic platform for lung cancer treatment.