Glioblastoma (GBM) remains highly lethal despite aggresive therapies, prompting interest in natural killer (NK) cell-based immunotherapy. However, efficient blood-brain barrier (BBB) delivery and GBM specificity remain key challenges for clinical translation. To address these limitations, we introduced a lipid-based polymer incorporating hyaluronic acid (HA) and glucosamine (Glc) onto NK cell surfaces via hydrophobic interactions through a simple 30-min incubatioin. This modification enabled outward presentation of Glc (a GLUT1 ligand) and HA (a CD44 ligand), enhancing interactions with GLUT1-overexpressing BBB endothelial cells and CD44/GLUT1-overexpressing GBM cells. As a result, surface-engineered NK cells efficiently transmigrated the BBB and selectively recognized GBM cells, leading to synergistic tumor elimination in an orthotopic GBM mouse model. Collectively, this study introduces a simple, non-genetic NK cell engineering strategy for clinically translatable GBM immunotherapy.