Glutathione displacement assay based on a fluorescent Au(I) complex
발표자
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초록
내용
Glutathione (GSH) is a crucial molecule for maintaining intracellular redox homeostasis and controlling a variety of pathophysiological processes. However, understanding the chemical mechanisms of GSH-induced processes is limited due to the lack of appropriate detection tools. To address this challenge, we have developed a fluorescent GSH probe based on a linear, homoleptic Au(I) complex with two 1,3-diphenylbenzimidazolium carbene ligands. The Au(I) complex produced a fluorescence turn-on response to GSH with a short response time of a few seconds. This rapid response was attributed to the displacement of the carbene ligand with GSH, involving a labile inner-sphere coordination interaction. Finally, we demonstrated the biological utility of our GSH probe by unambiguously discriminating between different GSH levels in normal and senescent preadipocytes.