Surface Stimuli-induced 3D Cardiac Spheroid Biochip
발표자
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초록
내용
Spheroids (cell aggregates) produced via three-dimensional (3D) cell culture methods have attracted an increasing amount of interest in the field of drug screening due to the significant advantages associated with more accurate simulation of their in-vivo behavior compared to two-dimensional culture methods. Conventional techniques for preparing spheroids include hanging drops, concave microwell aggregates, spinner flasks, and gravity cycling. However, all these methods have limitations such as low scalability, low viability, loss of spheroids, and irregular size distribution. To overcome these limitations, in this study, 3D cardiomyocyte spheroids were efficiently fabricated using biocompatible RGD peptide-coated silica nanoparticles (700 nm). In addition, we patterned RGD-coated silica arrays of various shapes and effectively controlled cardiac cell differentiation on the patterns.