Biomedical supramolecular materials can be designed and engineered at the molecular level to have specific functions and properties relevant to biological applications. These materials are formed through non-covalent interactions, such as hydrogen bonding, van der Waals forces, π-π stacking, and electrostatic interactions, which allow molecules to self-assemble into well-defined structures. The unique properties of supramolecular materials make them attractive for various biomedical applications due to their potential for controlled drug delivery, receptor stimulations, and molecular imaging. Here, I present the advances in biomedical materials using molecular assembly concerning the rational design of self-assembling aromatic amphiphiles, the formation of diverse nanostructures, and biological applications. Tamoxifen-selective fluorescent assembly, 2D peptide assembly, the transformation of the membranes to vesicles, and non-cytotoxic quercetin fluorophore are introduced.