Graphene oxide-incorporated metallic polymer nanopillar arrays for non-destructive electrochemical monitoring of dopamine release from living neurons
발표자
()
초록
내용
In Parkinson's disease (PD), where the loss or abnormal functionality of dopaminergic (DAnergic) neurons contributes to its pathogenesis, engrafting stem cell-derived neurons ex vivo has shown potential for partial recovery. However, accurately assessing the functionality of neurons remains a challenge. This study introduces a novel conductive cell cultivation platform, the graphene oxide-incorporated metallic polymer nanopillar array (GOMPON), capable of electrochemically detecting dopamine exocytosis from living neurons. Its excellent biocompatibility enables the cultivation and differentiation of model DAnergic neurons on the platform, facilitating real-time and non-destructive quantification of dopamine release. Through applying GOMPON, we demonstrate the precise assessment of functionality in DAnergic neurons derived from stem cells by electrochemically measuring dopamine exocytosis. The development of GOMPON represents a promising advancement with diverse applications.