Functionalized Hyaluronic Acid/Calcium Phosphate Hybrid-Nanoparticle for Efficient Gene Transaction of Human Mesenchymal Stem Cells toward Osteogenesis
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초록
내용
The clinical use of human mesenchymal stem cells (hMSCs) has rapidly expanded in regenerative medicine and tissue engineering due to their multi-lineage differentiation and self-renewal potential. However, their specific genetic regulation remains a challenge, particularly in directing them to differentiate into desired cell types in vivo. Therefore, a safe and efficient gene delivery system should be needed to manipulate the genetic levels involved in the differentiation of hMSCs. In this study, we utilized biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with catechol-derivatized hyaluronic acid (dopa-HA) to enhance hMSC differentiation through gene transfection. Dopa-HA/CaP demonstrated superior transfection compared to branched PEI (bPEI, MW 25 kDa) owing to a specific ligand-receptor interaction between HA and CD44 receptors on bone marrow-derived hMSCs. Furthermore, dopa-HA/CaP achieved significant improvement in the osteogenic differentiation of hMSCs through co-delivery of BMP-2 pDNAmiRNA-148b.