Polymeric Biomimetics of Cell-Penetrating Peptides for Energy-Independent Intracellular Gene Delivery
발표자
()
초록
내용
This study demonstrates efficient gene transfer into mammalian cells using small molecular amphiphile-polymer conjugates, specifically bile acid-polyethylenimine (BA-PEI). These conjugates provide an effective means for transporting genetic materials across the cell membrane without relying on endocytosis or other energy-consuming processes. The process mimics the macromolecular transduction observed with cell-penetrating peptides. The exposure of the hydrophilic face of the amphiphilic BA moiety on the surface of the BA-PEI/DNA complex is crucial in facilitating direct contact between BA molecules and the cell surface, thereby enabling an endocytosis- and energy-independent internalization process. This innovative approach using polymeric biomimetics has promising applications for enhancing the delivery of macromolecular therapeutics.