Surface modification of PDMS microfluidic chips to improve cell harvesting and nucleic acid detection
발표자
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초록
내용
PDMS microfluidic chips are widely used due to their physical properties, but their hydrophobic nature can limit certain applications. So we improved the ability of the PDMS microfluidic chip to harvest cells and detect nucleic acids by using Layer-by-Layer (LbL) multilayers of polyelectrolytes on the PDMS surface. The modified surface characteristics were confirmed through various techniques such as contact angle measurements, atomic force microscopy, cell culturing, and nucleic acid attachment. The deposition of multilayer films effectively enhanced the wettability and antifouling effects of PDMS microfluidic channels, enabling better cell culturing and nucleic acid detection. Moreover, by introducing a micropattern on the bottom surface using a photosensitive polymer, we were able to achieve controlled cell morphology. These findings suggest that polyelectrolyte multilayer films could be a promising surface modification strategy for biochips used in Point-of-care testing (POCT).