<u>Catalytic hairpin assembly (CHA)-based rolling circle amplification (RCA) assay for optical detection of miRNA</u>
발표자
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초록
내용
MicroRNA (miRNA), plays an important role in gene regulation in cancer cells, which recognized as a promising biomarker. In this study, we integrated CHA-based RCA assay and Zinc Finger-fused alkaline phosphatase (ZnF-Ap) to detect miRNA. In the presence of target miRNA, CHA cycle occurs and form DNA probe. And then circular DNA is attached to DNA probe, so that DNA probe can be used as a primer for RCA. The signal is amplified by the long single-stranded DNA synthesized through the RCA reaction, which has a large number of binding sites for ZnF-AP. After ZnF-AP is attached to long single strand DNA, the color change can be observed by using the chromogenic substrate with naked eyes. Using this analysis enables sensitive diagnosis even with very small amounts of target miRNAs and observation by changes of colors with naked eyes. Eventually, our study has potential to detect various miRNA, as a platform technology in miRNA diagnostic fields