Enhancing cardiomyocyte maturation by controlling the topographical bio-interface in the polymeric multi-scale hierarchical structure
발표자
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초록
내용
Topography of biomaterials is critical in cardiac tissue engineering. Thus far, single-scale topographies with a broad range of feature shapes and sizes have been reported. Here, we report the hierarchical topography composed of polymeric nano-pillars (nPs) on micro-wrinkles (µWs) for effective maturation of CMs. The topographical bio-interface is controllable in a vast size range and the responses of CMs are systematically investigated on it. Maturation of CMs on the hierarchical structures is highly enhanced compared to a single-scale topography: cardiac differentiation on the hierarchical topography is ≈ 3 times higher than those consisting of single-scale µWs and nPs. We revealed that the aspect ratio of nPs and the wavelength of µWs are important in CM maturation. This advance is caused by strong focal adhesion-mediated mechanotransduction confined within µWs and the cellular membrane protrusion on the nPs. We believe the hierarchical topography can advance the tissue engineering.