Effects of Substrate Composition and Mechanical Properties on Fibroblast Behavior
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This study explores the complex relationship between cells and the surrounding matrix, focusing on the influence of substrate composition and mechanical properties on fibroblast. Three distinct substrates were utilized: conventional plastic, collagen-coated plastic, and collagen hydrogel. Fibroblasts were cultured on these substrates in both two-dimensional and spheroid configurations. The study explored how substrate properties impact crucial cellular processes, including proliferation and migration. Moreover, we investigated the release profile of collagen and glycosaminoglycan. Immunocytochemistry analysis was performed to evaluate the expression of YAP and E-cadherin, exploring the dynamics of cellular signaling and adhesion. Our findings indicate that spheroid culture on soft collagen hydrogel substrates more closely mimics the natural cellular environment. These insights contribute to the understanding of cell-matrix interactions and hold implications for tissue engineering.