Reactive Polymer Based Near-Infrared Fluorescent Nanoparticles for Bioimaging
발표자
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초록
내용
Fluorescent molecules incorporating near-infrared (NIR) dyes are extensively employed for labeling biological targets in both in vitro and in vivo imaging applications. However, the utilization of NIR organic dyes as fluorescent markers presents two challenges. Firstly, the limited availability of binding sites on cells leads to low detectable intensity. Secondly, the hydrophobic nature of the dyes poses complication when operating in an aqueous environment. In this study, we demonstrate fluorescent copolymers by grafting amine-functionalized IR775 NIR dyes and poly(ethylene glycol) (PEG) chains to a reactive poly(pentafluorophenyl acrylate) (PPFPA) precursor polymer. The fluorescence properties of the copolymers and their self-assembly behavior in water are investigated. We further prepare biotinylated fluorescent copolymers with an optimized dye composition to illustrate in vitro cell viability and selective fluorescent labeling capability.