Fabrication and Characterization of L-threonine Polyurethane (LTHU) Nanoparticles for Drug Delivery System
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The L-threonine polyurethane (LTHU) for matrix of drug drlivery was synthesized using desaminotyrosyl L-threonine hexyl ester (DLTHE), poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PLA-PEG-PLA) and 1,6-hexamethylene diisocyanate (HMDI). The DLTHE was prepared based on the L-threonine hexyl ester (LTHE), which was pre-synthesized by esterification with hexanol. LTHU was designed to be degradable through the hydrolysis and enzymatic degradation of ester and amide linkages. Using W-O-W double emulsion method, LTHU nanoparticles were fabricated for encapsulating anticancer drugs such as carboplatin. All the synthetic processes were confirmed by ¹H-NMR, ¹³C-NMR and FT-IR spectroscopies, and molecular weights were confirmed by GPC. The morphologies of nanoparticles were characterized by DLS, FE-SEM and TEM and drug encapsulation was confirmed by confocal laser scanning microscope and fluorescence microscope. Drug loading efficiency and encapsulation efficiency were confirmed by UV/Vis spectroscopy.