Fabrication and Characterization of L-lysine Polyurethane (LPU) Nanoparticles for Drug Delivery System
발표자
()
초록
내용
To obtain biodegradable biomaterial for matrix of drug delivery, L-lysine polyurethane (LPU) was designed using the biodegradable triblock oligomer polylactide-poly(ethylene glycol)-polylactide (PLA-PEG-PLA), 1,6-hexamethylene diisocyanate (HMDI) and L-lysine ethyl ester (LEE). PLA-PEG-PLA was synthesized by ring opening polymerization (ROP) of lactide monomer onto poly(ethylene glycol) core. LEE was synthesized by esterification of L-lysine with ethanol using thionyl chloride as a catalyst. LPU nanoparticles were fabricated by using water-in-oil-in-water double emulsion technique for encapsulating anticancer drugs such as 5-fluorouracil (5-FU). The structure and composition of all synthesized materials was confirmed by 1H-NMR, 13C-NMR, FT-IR spectroscopies, and the molecular weights of polyurethane and PLA-PEG-PLA triblock were characterized by GPC analysis. The morphology of nanoparticles was observed by DLS, FE-SEM and TEM and drug encapsulation was confirmed by confocal laser scanning microscope.