Effects of Mineralized Fibers for Differentiation of Mesenchymal Stem Cells under 3D hydrogel Microenvironment Depending on the Origins of Cells
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초록
내용
Mineralized materials inducing osteo- or chondrogenic differentiation of mesenchymal stem cells (MSCs) have been used for tissue engineering, but the effects on MSCs from different origins within a 3D microenvironment have barely been investigated. We developed mineralized fibers incorporated within a spheroid with MSCs-derived from adipose (ADSC), umbilical cord (UCMSC), or bone marrow (BMSC), and then, encapsulated each composite spheroid within a gelatin methacryloyl hydrogel. The investigations of each composite spheroids-laden hydrogel proved that the ADSC, UCMSC, and BMSC spheroids displayed the longest sprouting, reticular-shaped sprouting, and spindly short sprouting, respectively. Moreover, chondrogenic and osteogenic differentiation were more favorable for the UCMSC and BMSC spheroids, respectively. Overall, our study highlights selecting appropriate cell types is important for designing mineral-based 3D osteochondral tissue models or transplants for regeneration.