Effect of Aggregation State of an Poly(2-hydroxyethyl methacrylate) Adsorbed Layer on Fibroblast Adhesion
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초록
내용
Aggregation states of polymer chains at the water interface strongly impact on various bio-related events such as protein adsorption and cell adhesion. In this study, we examined the aggregation states of an adsorbed layer of poly(2-hydroxyethyl methacrylate) (PHEMA) on a SiOx substrate in the underwater environment and its effect on the adhesion behavior of NIH3T3 fibloblasts. Neutron reflectivity measurements revealed that the PHEMA adsorbed layer was consisted of two slabs with different densities normal to the air interface. When contacting the PHEMA adsorbed layer with water, the outermost slab of the layer was swollen, whereas the underneath slab was almost unchanged in terms of the density. On such the PHEMA adsorbed layer, NIH3T3 cell adhesion was well suppressed, although its thickness was very thin less than approximately 20 nm. It was found that the PHEMA adsorbed layer showed excellent bioinertness properties in the aqueous phase.