Surface functionalization of 3D printed scaffold with minerals and pro-angiogenic growth factor for vascularized bone regeneration
발표자
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초록
내용
Surface engineering of scaffolds have been widely utilized for bone tissue regeneration. However, inducing regeneration of complex-structured bone tissue still remains a challenge. Thus, we introduced minerals and PDGF to the surface of scaffolds. First, the surface of scaffolds were homogeneously mineralized by polyphenol mediated SBF mineralization with 0.01 M sodium bicarbonate (M2). The M2 scaffold demonstrated high mineral ions, mechanical property and osteoconductivity. Then, we immobilized PDGF on M2 scaffold (M2/P) to improve cell interaction and induce the cells to release pro-angiogenic growth factors. Finally, in vivo implantation of M2/P scaffold onto a mouse calvarial defects demonstrated 80.8 ± 9.8% regeneration area and 10 ± 2 newly formed arterioles for each sample, which were significantly enhanced. In conclusion, our functionalized scaffolds with biomolecules could be used to clinically improve the regeneration of complex structured bone with vascularization.