Korea-Japan Joint Symposium: Emerging Biopolymers for Advanced Applications (English)
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Development of Self-assembling Peptide Tags for Intracellular Reconstitution of Protein Assembly
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In cells, a wide variety of proteins assemble to express their functions. To reveal these versatile biological processes, techniques to rationally reconstitute protein assemblies in cells are required. For this purpose, we have developed a Y15 self-assembling peptide tag.1 Y15 peptide, consisting of alternative repeats of hydrophobic Tyr and hydrophilic Glu and Lys, self-assembles into a nanofibril structure. By genetically tagging Y15 to proteins, we successfully constructed protein assemblies in cells. Furthermore, we reconstructed the Nck1 protein assemblies initiating the actin polymerization reaction in the cell and analyzed the effects of the composition on the protein-protein interaction2 and reaction. (1) T. Miki et al., Nat. Commun., 12, 3412 (2021). (2) T. Miki et al., Chem. Commun., 57, 11338 (2021).