Bioprinting cell-laden tissue constructs using visible light crosslinkable bioinks
발표자
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초록
내용
Bioprinting is a rapidly developing technique to fabricate functional tissues and organs. Among many bioprinting techniques, photocrosslinking based bioprinting allows to prepare 3D complex hydrogels with tissue-specific properties. However, poor biocompatibility and low photoreactivity of existing bioinks limit their use in the fabrication of cell-laden 3D tissue constructs. Here, we report a fast-curable low-energy bioprinting system using a photosensitive hyaluronic acid (HA) and water-soluble photoinitiator (PI) for visible light-induced 3D bioprinting. After examining the water solubility, molar extinction coefficients, photocrosslinking efficiency and biocompatibility of the PIs, corneal structure was fabricated and the cell viability in the 3D-printed constructs were evaluated. Overall, the HA-based bioinks exhibited superior properties to function as a fast-curing visible light-induced 3D printing material suitable for cell-laden tissue constructs for tissue engineering.