Surface charge-mediated nucleic acid extraction and purification using a positively-charged copolymer synthesized via iCVD
발표자
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초록
내용
The rapid and sensitive diagnostics of viral infection throughout nucleic acid-based methods, such as PCR, has been significant as the viruses are spreading on a global scale. However, the detection methods rely on extracting the nucleic acid from the patients, which requires multiple liquid handling and centrifugation steps. Herein, we develop a single-step RNA extraction method using charge interaction between the nucleic acid and positively charged copolymer synthesized via iCVD. A tertiary aminated DMAMS was copolymerized with varying degrees of epoxy-containing GMA to design the copolymer. Successful copolymerization was confirmed by FTIR, XPS, and contact angle measurement. The series of copolymer films were deposited on the surface of the PCR tube and applied to bind and isolate nucleic acid through the charge interaction covalently. The contaminants, including proteins and cellular components, were reacted with GMA and assisted nucleic acid purification from the specimen.