High-throughput screening of Yarrowia Lipolytica producing higher growth and lipid accumulation using pneumatic bilayer valve-based microfluidic sorter
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Biofuels derived from microalgae showed promising potential as a future renewable bioenergy source. Here, we present a microfluidic droplet sorter (μFADS) for screening randomly mutated Yarroiwa Lipolytica library and sort out the target subsets exhibiting higher growth rate and increased lipid production First, cell-encapsulated alginate droplets are generated within the flow-focusing device, followed by alginate gelation through calcium release by lowering pH. The acetic acid in the continuous phase diffused into the droplet due to the difference in solubility, resulting in pH decrease. Then, the Y. Lipolytica cells were cultured in alginate gels that functioned as an individual micro-reactor and re-injected into μFADS to evaluate their capacity and sort target cells. As a result, the alginate gels containing cells showing 1.5 – 2 times higher growth and lipid production rate were selectively retrieved compared to the cells in the waste channel.