Synthesis and Characterization of Guanidinylated Polyethylenimine-Conjugated Hydroxyethyl cellulose for Gene Delivery System
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In this work, guanidinylated polyethylenimine-conjugated hydroxyethyl cellulose (HECP2kG) was synthesized for gene delivery system. Hydroxyethyl cellulose (HEC) is widely used for variety applications due to its water solubility and biocompatibility. Introducing guanidine moieties is an efficient method for enhancing cellular uptake since guanidine groups are important components of cellular penetrating peptides. After synthesizing polyethylenimine functionalized hydroxyethyl cellulose (HECP2k) by reductive amination of low molecular weight polyethylenimine (PEI, Mw 2000) to oxidized HEC, HECP2kG was finally obtained through guanidinylation using DIPEA and 1HPC. HECP2kG could form positively charged nano-particles with pDNA. MTT assay and luciferase transgene expression assay was conducted to examine cytotoxicity and transfection efficiency. As a result, cytotoxicity and gene delivery efficiency of HECP2kG were investigated in comparison with HECP2k.