MOF nanoparticles with ice surface-microcurvature control for efficient cell-cryopreservation
발표자
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초록
내용
Ice binding proteins (IBPs) have been recognized as cryoprotectants (CPAs) for the survival of organisms in sub-zero conditions because of lowering the freezing point of water and ice recrystallization inhibition (IRI) by binding to the growing ice surface. CPAs developed by mimicking these properties use the interaction with ice to prevent the cryoinjury of cells or tissues. Herein, zirconium (Zr)-based MOF-801 nanoparticles (NPs) with high biocompatibility were used as CPAs in an aqueous solution. The smallest NP induces a high micro-curvature of the ice surface, showing a higher IRI effect. Surface-functionalization of MOF-801 NPs with ice-binding pendants confer hydrophilic/hydrophobic duality to the NPs, resulting in hydrogen bonding with ice and preventing water molecules from assimilating with the ice surface, leading to superior cell CPAs efficiency. This study suggests low-cost, high-dose MOF NPs CPA for organ/cord blood preservation, and vaccine/pharmaceutical applications.