Binary nanoparticles-graphene hybrid structure based magnetofluoro-immunoassay for detection of cancer cell derived exosome biomarker
발표자
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초록
내용
A multi-functionalized carbon nanomaterial has been highlighted due to its amazing synergic properties such as PRET, magneto-optical effect, SERS and so on. And in this study, silver nanoparticle (Ag NP) and iron oxide nanoparticle (IONP) decorated graphene (GRP) was prepared in D.I water and utilized for biosensing platform. In this case, Ag NP and GRP have the plasmonic property and IO NP has magnetic property, thus, this hybrid nanomaterial could play a role as magentoplasmonic substrate for magnetofluoro-immunoassay. On the other hands, recently, exosome has been considered as potential biomarkers for diagnosis of diseases. In this study, prostate cancer cell (PC; LNCaP.FGC) derived exosome was successfully detected by using Ag/IO-GRP with assistance of dye-tetraspanin antibody. According to the amount of exosome, the FL intensity would be changed and this system showed high sensitivity and selectivity. Thus, this hybrid material showed excellent potential for biosensing platform.