Hyaluronated Lipid Nano Particle for mRNA Vaccines
발표자
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초록
내용
In the era of COVID-19, lipid Nano Particle (LNP) has become an important platform for the development of mRNA vaccines. However, the current clinical LNP using poly(ethylene glycol) for mRNA vaccines has been known to cause side effects in a few patients. Here, we developed a hyaluronate (HA) conjugated LNP for mRNA vaccine delivery. Several cationic lipids were conjugated to HA and the resulting HA-LNP conjugates were used to make a complex with eGFP expressing mRNA as a model vaccine. In vitro, HA-LNP/eGFP complex showed the high expression of eGFP by HA receptor mediated endocytosis. In addition, we developed HA-mRNA conjugate to make a complex with LNP for mRNA vaccines. This presentation will address the feasibility of HA-based LNP for the pulmonary delivery of mRNA vaccines.