DNA-Immobilized Fluorescent Polystyrene Nanoparticles as Biosensors with Tunable Detection Limits
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초록
내용
Dye-loaded polymer nanoparticle-based nucleic acids detection systems have been reported in recent years because of flexibility in tuning emission properties. They are commonly prepared by nanoprecipitation, the quantitative control over the number of immobilized dyes is difficult and additional crosslinking is required to minimize dye leakage. Herein, sensor nanoparticles were prepared through controlled surface reactions of immobilizing dye molecules and capture DNAs on polystyrene nanoparticles. The off-state of the sensor is achieved by the hybridization of quencher-functionalized DNA, then target DNA is detected through a strand displacement strategy. We find that surface chemistry allows for quantitative control of immobilized fluorescent dyes and DNA strands. By adjusting the dye to capture DNA ratio, the detection limit can be tuned. This new design offers sensor with tunable detection profiles, which we envision can find utility in a wide range of biosensing applications.