Multiplex DNA library purification by synthesis and selection
발표자
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초록
내용
Nucleic acids can be designed and assembled into biological systems in a programmable manner to create functionalities unseen in nature. As a result, Complex oligonucleotide libraries are essential materials for various biotechnological applications, including synthetic biology and therapeutics. However, conventional purification methods, which mainly focus on purifying single species, could not be applied to complex oligo libraries to lower the error rate after synthesis. Since most errors that occur during the synthesis are deletions, I developed Multiplex oligonucleotide libraries purification by synthesis and selection (MOPSS), a length-based purification method with single-base resolution. With an automized system for coupling the intended number of nucleotides to the oligos, libraries encoding the complementarity-determining regions H3 (diversity ＞ 109) were simultaneously purified in one pot, and the in-frame oligo ratio was increased from 49.6% to 83.5%.