Fibroblast spheroid culture on the stiffness-controllable collagen hydrogel substrates
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초록
내용
The mechanical property of cellular substrates affects the cell behaviors such as proliferation, migration, and differentiation. Researchers have evaluated the effects of substrate stiffness on cellular behaviors using conventional 2D cell culture. In this study, we cultured the fibroblast spheroids on the collagen hydrogel substrate and compared them with the traditional 2D cellular culture.MTT assay, collagen, and GAGs quantification were evaluated to compare the differences. The 3D spheroid culture on the stiffness-controllable matrix can present more useful results because it is closer to the actual cellular environment than the 2D culture.