Effect of Cell-Derived Extracellular Matrix on Macrophages Behavior and Wound Healing
발표자
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초록
내용
In this study, we prepared cell-derived matrix (CDM) from four different cell sources and selected human lung fibroblast derived matrix (hFDM) and umbilical cord-blood mesenchymal stem cell derived matrix (UMDM) for the study of ECM-macrophage interactions. When THP-1 cells-derived macrophages (M0) were seeded on the UMDM or hFDM, the M0 on UMDM presented more diverse cytokines release profile. Conditioned medium obtained from macrophages grown on UMDM showed significantly better wound closure via human dermal fibroblast scratch assay. For in vivo study, we fabricated a composite hydrogel containing each CDM and Pluronic/hyaluronan, and applied it on deep wound model. Histological analysis revealed that UMDM-treated group led to more effective wound healing, as proven via significant recovery of the skin appendages, better neovascularization, and larger number of macrophages (M2) at 7 days. Taken together, CDM should be a promising resource toward advanced wound healing.