Neural interfacing biomaterials surface-functionalized with neural specific lipid bilayers
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초록
내용
We hypothesized that probe supported with a cellular membrane possessing cell adhesion molecules (CAM) such as L1cam can promote neuronal adhesion and growth. For the fabrication of this bio-mimetic system, PC12 cells were differentiated into neural cells to induce the expression of the neural adhesive L1cam that can promote neurite outgrowth and impede astrocyte growth. The modified substrate has the enhanced air stability by tethering lipids on the surface of titanium and introducing the extracted membrane components by vesicle fusion method to form a tethered lipid bilayer. The successful surface modification was verified by FT-IR, confocal microscopy and FRAP assay. The modified titanium was resistant to protein adsorption. In vitro cell study confirmed that the natural tethered lipid bilayer (PM-TLB) provides highly biocompatible surface for neuron cells, in contrast limits the adhesion and proliferation of astrocyte cells.