Comparative study of different tethering of supported lipid bilayers surface coating of implantable biosensing devices
발표자
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초록
내용
Supported lipid bilayers membranes are growing interest as models of biological membranes for coating of implantable biosensing devices. Because of the limitation of bilayers which directly supported on glass are separated from the substrate by a thin film of water, bilayer formation on the tethered surface is the choice. Here, we prepared coated gold substrate with tethered lipid bilayer. Firstly, gold coated glass substrates were tethered by different types of thiol lipids. Secondly, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) was used for the formation of supported lipid bilayers. The successful surface modification was verified by FT-IR, confocal microscopy and FRAP assay. Antifouling assay was conducted with quartz crystal microbalance with dissipation (QCM-D) apparatus and fluorescence microscopy. The result demonstrates the successful formation of tethered lipid bilayer on gold substrate and provides suitable coating system of implantable biosensing devices