Lipid bilayers infused with a nitrated lipid tethered on titanium for the intra-vascular implantation
발표자
()
초록
내용
In the present study, we investigate the potential use of tethered lipid bilayer (tLB) membranes infused with a nitrated lipid generating nitric oxide (NO) for the sustained release of NO from tLB membrane-anchored on a metal surface. The NO generating tLB membranes are produced via following two steps: 1) immobilization of phospholipids on a metal surface and 2) fusing liposomes containing nitrated lipid with the pre-tethered phospholipids on the surface. Our data demonstrated that the tLB membrane was successfully anchored on a titanium surface. The tLB membrane dramatically reduce the adsorption of non-specific protein binding compared with a bare titanium plate. The lipid bilayer released NO up to 24 hours. In addition, NO released from the tLB membrane enhanced the viability of endothelial cell cultured on the surface of tLB membrane in vitro. Together, our results pave the way for the potential use of tLB-coated surfaces in biomedical devices for therapeutic purposes.