Endothelial cell membrane infused with a nitric oxide generating catalytic lipid and its tethering on titanium for the endo-vascular implantation
발표자
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초록
내용
The present study was developed to investigate the potential use of lipid bilayer-coated titanium containing membrane extracted from endothelial cells (ECs) and phospholipid with a catalytic selenium molecule producing nitric oxide (NO) with endogenous NO precursors. We fused selenium containing molecule into membrane from ECs and sequentially inserted membrane into pre-tethered phospholipids on the titanium surface. The tethered lipid bilayer exhibited endothelium-mimetic properties and successfully generated NO. When three types of cells including EC, smooth muscle cell, and macrophage were cultured on lipid bilayer, lipid bilayer played a role in a promotive, suppressive, and immunomodulatory manner to each cell, respectively. Together, our results not only provide understanding of the characteristics of catalytic NO-releasing lipid and cellular responses to lipid bilayer but also highlight the development of versatile design for vascular implantation and bio-interfacing devices.