Novel Kanamycin-PEI as a new class dendrimer for gene delivery
발표자
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초록
내용
Recently, non-viral vectors increased the attention because of their advantages. In this study, the kanamycin-methylacrylate-low molecular weight of polyethylenimine (KMP) library was prepared as gene carriers. Kanamycin can be hydrolysed under acidic environment. Hence, KMP 0.6 kDa, KMP 1.2 kDa and KMP 2 kDa were synthesized to determine the best gene carriers with minimize toxicity. KMPs were confirmed 1H NMR, FT-IR and buffering capacity after synthesised. Gel electrophoresis and picogreen assay have been studied to evaluate the ability to bind on DNA. The polyplexes have nanosize and positive charge. The cytotoxicity and transfection have been evaluated using human cervical carcinoma (HeLa) and human liver carcinoma (HepG2) cell line. The data implied that KMPs have lower toxic compare to PEI 25 kDa, and higher transfection compare to original low molecular weight of PEI. KMP 2 kDa and KMP 1.2 kDa are good carriers.