The development of poly(amidoamine) derivative using NLS in classical protein import.
발표자
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초록
내용
Vectors used in gene therapy products include viral and non-viral vectors, but non-viral vectors are limited in use because their target efficiency is lower than viral vectors. We synthesized poly(amidoamine) and amino acid sequences that have less toxicity and can increase gene transfection efficiency. Classical NLS (nuclear localization sequence) has the characteristics of a monopartite containing basic amino acid residues lysine and arginine as a single cluster. By optimizing the certain sequence, it was synthesized with PAMAM using two sequences, KKRK and KKRKH. The synthesis rate is calculated by NMR analysis, and the Zeta potential is used to measure the particle’s charge and particle size via DLS. The cell line was confirmed to have higher transfection efficiency and lower toxicity than the control by making complex with pDNA using Hep G2 and NIH-3T3. The visual data through the confocal image show that the polymer was well introduced into the cell.