ATP recognition based on the FRET from organic semiconducting molecules to Cy3 fluorophores
발표자
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초록
내용
Adenosine triphosphate as a major source of chemical energy in living organisms can drive many processes in living cells. In the previous researches, ATP recognition is playing a critical part in cell biology and clinical diagnostics. However, traditional methods, such as chromatographic, electrochemical and bioluminometric approaches are time-consuming and involved in complex chemical or enzymatic reactions. In this research, hybrid-assemblies has been fabricated which composed of double strands DNA and organic semiconducting tri (8-hydroxyquinoline) aluminium (Alq3) molecules. One of the single-strand DNA molecules which labeled with the Cy3 fluorophore can specifically recognize ATP as an aptamer. Under this condition, Förster resonance energy transfer (FRET) from Alq3 to Cy3 can be observed. However, after adding ATP, owing to strong affinity with ATP, Cy3 labeled aptamer release from the hybrid-assemblies that induced the decreasing of FRET.