FRET-Nanomaterials for One-Step Sensors of Cellular Cholesterol
발표자
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초록
내용
Cholesterol is an important component of animal cell membranes and the main precursor for synthesis of different biomolecules such as bile acids, steroid hormones and vitamin D. Various analytical methods have been developed for detecting cholesterol which exists in lipoprotein form mixed with lipid and proteins in serum. On the other hand, the conformation of cholesterols varies depending on the environment in which it is surrounded. In our research, we try to detect a cholesterol which is embedded in cell membrane to figure out a relationship between lipid and cholesterol in membrane. Turn-on sensing mechanism based on FRET is suitable for targeting a low concentration of cholesterol in cell membrane because of its high sensitivity. We developed one-step fluorescence turn-on sensing materials with combination of commonly used nanomaterials (AuNPs, Au Nanocube, GO) and chem-bio ligand interfacing. It can be observable to detection of cholesterol in cell membrane.