pH-Triggered Skin Tissue Sealing Using Cellulose Nanofibrils-Reinforced Hydrogel Bioadhesives
발표자
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초록
내용
This study introduces a mechanically reinforced mussel-mimetic skin tissue sealing system in which a compoite hydrogel bioadhesive consisting of cellulose nanofibers (CNFs) and boronic acid-conjugated alginate (Al-BA) was employed. Boronic acid has a cis-diol moiety that exhibits pH-responsive curing from a viscoelastic solution to a soft gel under physiological conditions. However, Al-BA itself has a limitation to tissue suturing due to formation of intrinsic soft gel phase. Therefore, it is necessary to develop a technology to reinforce the gel phase which has suitable adhesion strength for tissue sealant. For this, we conjugated 3-aminophenylboronic acid onto an alginate backbone via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride /N-hydroxysuccinimide reaction, and then hybridized with CNFs. Finally, we demonstrated that our bioadhesives exhibited effective skin tissue sealing, thus promising biological applications in the field of tissue engineering.