Cell Spheroid Culture System with Porous Microparticle for Prolonged Cell Survival and Enhanced Osteogenic Differentiation
발표자
()
초록
내용
Development of three-dimensional (3D) cell spheroid systems is considered as a promising technology to mimic in vivo microenvironment compared to monolayer-culture. However, limited size of spheroid due to the insufficient supply of oxygen/nutrients into the central region is still remained as a critical huddle. In this study, we developed microparticles with leaf-stacked structure (LSS particles) which can allow sustained release of bioactive molecules as well as provide space for sufficient supply of oxygen/nutrients. The cell spheroid was prepared using the LSS particles and human bone marrow-derived mesenchymal stem cells (hBMSCs). The morphology of LSS particles and cell/LSS spheroid was observed by a scanning electron microscope (SEM) and release behavior of BMP-2 from LSS particles was determined using a ELISA kit. Cell survival/structural stability/osteogenic differentiation (in vitro) and new bone formation (in vivo) of cell/LSS spheroid system were investigated.