Enhanced Cellular Uptake of Surface-Functionalized Polymer Nanoparticles
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초록
내용
Functional polymer nanoparticles have been widely exploited for the treatment of various diseases, including obesity. In this study, we hypothesized that the adipocyte uptake of polymeric nanoparticles could be enhanced using cell-penetrating peptide (CPP). Poly(lactic-co-glycolic acid) (PLGA) nanoparticles containing calcium carbonate were fabricated by the double emulsion method as gas-generating polymer nanoparticles, and cell-penetrating peptide (CPP) was then covalently coupled to the surface of the nanoparticles via carbodiimide chemistry. Various characteristics of PLGA nanoparticles, including size, size distribution, loading content of calcium carbonate, cytotoxicity, and intracellular uptake were investigated in vitro. This approach of CPP-mediated delivery of functional polymer nanoparticles may be useful in many therapeutic applications.