Dual-crosslinkable self-healing hydrogel for 3-D bioprinting
발표자
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초록
내용
Three-dimensional(3-D) bioprinting has developed as an important tool for tissue regeneration. Hydrogels for 3-D bioprinting should have low viscosity that can be printed efficiently but simultaneously can form solid or semi-solid structure. Herein, dual cross-linkable hydrogels with self-healing ability are proposed for 3-D bioprinting. Oxidized hyaluronate(OHA), glycol chitosan(GC) and adipic acid dihydrazide(ADH) can form self-healing hydrogel via competitive reaction between imine bond(OHA/GC) and acylhydrazone bond(OHA/ADH). Hyaluronate-alginate hybrid(HAH) polymer was added to the self-healing gel for dual-crosslinking with calcium ions after the printing process. Dual-crosslinking significantly enhanced the stability of printed 3-D constructs and maintained their structure. Various characteristics of OHA/GC/ADH/HAH hydrogels including the mechanical properties, stability, chondrogenic differentiation of chondrocytes encapsulated within the gels were investigated in vitro.